Label-Free, Real-Time Monitoring of Biomass Processing with Stimulated Raman Scattering Microscopy

被引:75
|
作者
Saar, Brian G. [1 ]
Zeng, Yining [2 ,3 ]
Freudiger, Christian W. [1 ,4 ]
Liu, Yu-San [2 ,3 ]
Himmel, Michael E. [2 ,3 ]
Xie, X. Sunney [1 ]
Ding, Shi-You [2 ,3 ]
机构
[1] Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA
[2] Natl Renewable Energy Lab, Biosci Ctr, Golden, CO USA
[3] Oak Ridge Natl Lab, Bioenergy Sci Ctr, Oak Ridge, TN USA
[4] Harvard Univ, Dept Phys, Cambridge, MA 02138 USA
关键词
biofuels; cellulose; delignification; nonlinear optics; Raman spectroscopy; LIGNOCELLULOSIC BIOMASS; BLACK SPRUCE; CELL-WALLS; PRETREATMENT; BIOFUELS;
D O I
10.1002/anie.201000900
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
(Figure Presented) Label-free "chemical movies" of lignin and cellulose in fresh plant tissue were recorded during a step in the breakdown of biomass to produce biofuels by using stimulated Raman scattering microscopy. The technique allows the degradation of lignin in the cell wall to be monitored with high sensitivity, sub-micrometer spatial resolution, and high temporal resolution. © 2010 Wiley-VCH Verlag GmbH S. Co. KGaA, Weinheim.
引用
收藏
页码:5476 / 5479
页数:4
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