Development of a real-time PCR method for Thalassiosira rotula rapid detection

被引：0
|
作者：
He Shanying
Yu Zhigang [1 ]
Mi Tiezhu
机构：
[1] Ocean Univ China, Coll Chem & Chem Engn, Qingdao 266003, Peoples R China
[2] Ocean Univ China, Coll Environm Sci & Engn, Qingdao 266003, Peoples R China
来源：
ACTA OCEANOLOGICA SINICA | 2007年 / 26卷 / 02期
关键词：
red tide; Thalassiosira rotula; fluorescent quantitative PCR; 18S rDNA;
D O I：
暂无
中图分类号：
P7 [海洋学];
学科分类号：
0707 ;
摘要：
Gene specific primers and DNA probe were designed based on the sequence of 18S rDNA cloned from the red tide alga Thalassiosira rotula. A real-time fluorescent quantitative PCR (RFQ - PCR) method was developed for quantitative detection of T. rotula. The RFQ - PCR assay data showed that the results obtained with the RFQ - PCR quite good agreement with those with the light microscope (LM) counting method, which suggested that the RFQ - PCR could be a useful method for red tide alga detection.
引用
收藏
页码：133 / 139
页数：7
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