INDUCTION, GROWTH AND PACLITAXEL CONTENT OF NEEDLE-AND PETIOLE-DERIVED CALLI IN HIMALAYAN YEW (TAXUS WALLICHIANA ZUCC.) UNDER LIGHT-EMITTING DIODES

被引：1

作者：

Duong Tan Nhut ^{[1
]}

Phan Le Ha Nguyen ^{[1
]}

Nguyen Trinh Don ^{[1
]}

Hien, N. T. T. ^{[1
]}

Nguyen Phuc Huy ^{[1
]}

Nguyen Ba Nam ^{[1
]}

Bui The Vinh ^{[2
]}

Tran Cong Luan ^{[2
]}

机构：

[1] Vietnam Acad Sci & Technol, Tay Nguyen Inst Sci Res, Dalat City, Lam Dong Provin, Vietnam

[2] Ho Chi Minh City Res Ctr Ginseng & Med Mat, Ho Chi Minh City, Vietnam

来源：

ACTA BIOLOGICA CRACOVIENSIA SERIES BOTANICA | 2014年 / 56卷 / 02期

关键词：

10-Deacetylbaccatin III; callus; high-performance liquid chromatography; light-emitting diodes; paclitaxel; Taxus wallichiana Zucc; 1ST TOTAL-SYNTHESIS; IN-VITRO GROWTH; CELL-CULTURES; SUSPENSION-CULTURES; ENDOPHYTIC FUNGUS; TAXANE PRODUCTION; RED-LIGHT; TAXOL; CUSPIDATA; QUALITY;

D O I：

10.2478/abcsb-2014-0026

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

To achieve a better understanding of accumulation of paclitaxel and related taxanes in Himalayan yew (Taxus wallichiana Zucc.) callus mass, induction and growth of calli from needles and petioles under light-emitting diodes (LEDs) and production of paclitaxel and baccatin were investigated. Call were induced from needles and petioles on Gamborg's B5 medium supplemented with 1 mg.1(-1) kinetin and 4 mg-l(-1) 2,4-D under different combinations of blue and red LEDs. Calli were further cultured in that medium under the same lighting conditions. Callus induction from needles and petioles and growth was better under blue LEDs than under red LEDs and fluorescent light. The combinations of blue and red LEDs produced variable results. The paclitaxel content of these calli was quantified by high-performance liquid chromatography. The paclitaxel content of calli derived from needles was 0.00628% and the 10-DAB (10-Deacetylbaccatin III) content was 0.00366%. The paclitaxel content of calli derived from petioles was 0.00412%, and no 10-DAB was detected.

引用

页码：107 / 114

页数：8