New microsatellite markers developed from reported Ipomoea trifida sequences and their application to sweetpotato and its related wild species

被引：18
|
作者：
Hu, JJ [1 ]
Nakatani, M
Lalusin, AG
Fujimura, T
机构：
[1] Univ Tsukuba, Doctoral Program Agr Sci, Tsukuba, Ibaraki 3058572, Japan
[2] Ocean Univ China, Div Life Sci & Technol, Qingdao 266003, Peoples R China
[3] Natl Inst Crop Sci, Tsukuba, Ibaraki 3058518, Japan
[4] Univ Tsukuba, Doctoral Program Life & Environm Sci, Tsukuba, Ibaraki 3058572, Japan
[5] Univ Tsukuba, Inst Agr & Forest Engn, Tsukuba, Ibaraki 3058572, Japan
来源：
SCIENTIA HORTICULTURAE | 2004年 / 102卷 / 04期
关键词：
microsatellites; Iponioea trifida; sweetpotato; database; EST;
D O I：
10.1016/j.scienta.2004.04.014
中图分类号：
S6 [园艺];
学科分类号：
0902 ;
摘要：
Ipomoea trifida belongs to the family Convolvulaceae and is closely related to sweetpotato. In this study, we reported the microsatellites in I. trifida sequence database, and tested their transferability and polymorphisms for both sweetpotato and its related wild species. In the DNA database, 1425 sequences were registered for I. trifida. Sixty-one independent sequences were found to have microsatellite motifs and PCR primers were designed to amplify 15 microsatellites loci identified. Twelve primer pairs could amplify the expected product size and nine primer pairs showed polymorphisms among the three genotypes of L trifida. These 12 functional primer pairs were used to assess the transferability and the level of polymorphism between sweetpotato cultivars and its related wild species. The transferability showed, 100% for I. batatas, 83.3% for I. tiliacea, 75% for I. triloba and 66.7% for I. Lacunosa, respectively. These markers also revealed high level of polymorphism between wild species and sweetpotato cultivars. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码：375 / 386
页数：12
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