Rapid, Reliable, and Inexpensive HLA-B*58:01 Detection Method Using DNA Binding Dye-based Duplex Allele-specific Melting Curve Analysis

被引：0

作者：

Huh, Ji Young ^{[1
]}

Han, Ok Jin ^{[1
]}

Park, Geon ^{[2
]}

机构：

[1] CHA Univ, CHA Bundang Med Ctr, Dept Lab Med, Gyeonggi Do, South Korea

[2] Chosun Univ, Dept Lab Med, Coll Med, 365 Pilmun Daero, Gwangju 61453, South Korea

来源：

ANNALS OF CLINICAL AND LABORATORY SCIENCE | 2018年 / 48卷 / 03期

关键词：

Allopurinol hypersensitivity; HLA-B*58:01; Severe cutaneous adverse reactions; Melting curve analysis; DNA-binding dye; CUTANEOUS ADVERSE-REACTIONS; COST-EFFECTIVENESS ANALYSIS; TOXIC EPIDERMAL NECROLYSIS; STEVENS-JOHNSON-SYNDROME; ALLOPURINOL-INDUCED HYPERSENSITIVITY; CHRONIC RENAL-INSUFFICIENCY; HLA-B; ASSOCIATION; GUIDELINES; ASSAY;

D O I：

暂无

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Background. Allopurinol is the most commonly used drug for the treatment of gout and also one of the most common causes of severe cutaneous adverse reactions (SCARs). Human leukocyte antigenB*58:01 (HLA-B*58:01) is strongly associated with allopurinol-induced SCARs. The aim of the present study was to develop and validate a rapid and economic screening method for HLA-B*58:01. Methods. The accuracy of duplex allele-specific melting curve analysis using DNA-binding dye for HLA-B*58:01 was evaluated in 150 blood samples with sequence-based typing (SBT) as the reference method. Results. Fifty HLA-B*58:01-positive and 100 negative results obtained by duplex allele-specific melting curve analysis were completely in agreement with the SBT results. Conclusion. Duplex allele-specific melting curve analysis is a rapid, reliable and inexpensive assay that is appropriate for screening for the HLA-B*58:01 allele.

引用

页码：296 / 300

页数：5

共 8 条

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