Urinary stone is a worldwide disease and percutaneous nephrolithotomy (PCNL) is the most commonly used operation for staghorn calculi. Research indicated that SFR for staghorn calculi in one stage of PCNL showed 50%-91% results, under standard operation, standard instrument and professional treatment route. The present study aimed to explore the clinical factors of stone free rates (SFR) after PCNL for staghorn calculi. This retrospective study included 527 patients with staghorn calculi after one stage single channel PCNL. Associated factors with postoperative SFR after PCNL for staghorn calculi were analyzed using logistic regression model. The total SFR was 68.9% (363/527) in this group. 164 patients were diagnosed as residual stone after operation. In multivariate analysis suggested that factors of whether branched renal pelvis or not (OR=5.369, 95% CI: 0.957 similar to 30.109, P=0.056), Maximum stone diameter (OR=1.812, 95% CI: 1.088 similar to 3.016, P=0.022), minimum ratio of neck length to width renal transverse diameter (OR=0.002, 95% CI: 0 similar to 0.214, P=0.008), Average neck length (OR=5.842, 95% CI: 1.019 similar to 33.501, P=0.048). In conclusion, there are clinically significant factors of PCNL for staghorn calculi. Through logistic regression model, factors were correlated and preliminarily evaluated for the SFR after phase 1 operation, which intensely contributed to develop the individual medical programs.