Integrated Transcriptome and Metabolome Analysis Reveals an Essential Role for Auxin in Hypocotyl Elongation during End-of-Day Far-Red Treatment of Cucurbita moschata (Duch. Ex Lam.)

被引：3

作者：

Liu, Qi ^{[1
,2
]}

Zhang, Hanqing ^{[3
]}

Mei, Yanhao ^{[1
,2
]}

Li, Qi ^{[1
,2
]}

Bai, Yahui ^{[1
,2
]}

Yu, Huibin ^{[1
,2
]}

Xu, Xiaodong ^{[1
,2
]}

Ma, Jianxiang ^{[1
]}

Wu, Yongjun ^{[3
]}

Yang, Zhenchao ^{[1
,2
]}

机构：

[1] Northwest A&F Univ, Coll Hort, Yangling 712100, Shaanxi, Peoples R China

[2] Northwest A&F Univ, Key Lab Protected Hort Engn Northwestern China, Minist Agr & Rural Affairs, Yangling 712100, Shaanxi, Peoples R China

[3] Northwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China

来源：

AGRONOMY-BASEL | 2021年 / 11卷 / 05期

关键词：

Cucurbita moschata (Duch. ex Lam.); far-red light; auxin; transcriptome; metabolomics; SHADE AVOIDANCE; LIGHT QUALITY; ALPHA-SUBUNIT; PLANT-GROWTH; ARABIDOPSIS; TRYPTOPHAN; BIOSYNTHESIS; TRANSPORT; RESPONSES; PATHWAY;

D O I：

10.3390/agronomy11050853

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Long, robust hypocotyls are important for facilitating greenhouse transplant production. The use of far-red light at the end of the day (end-of-day far-red, EOD-FR) is known to prompt hypocotyl elongation, but the mechanism of EOD-FR-mediated hypocotyl elongation in pumpkin remains unclear. Here, we found that hypocotyl length, parenchymal cell size in hypocotyls, and plant IAA levels were significantly greater in pumpkin after EOD-FR treatment. This effect was counteracted by the application of the polar auxin transport inhibitor 1-N-naphthylphthalamic acid. Integrated transcriptomic and metabolomic analysis of pumpkin hypocotyls revealed that the expression of auxin-related genes changed significantly after EOD-FR treatment, and the contents of the auxin biosynthetic precursors tryptophan and indole were also significantly higher. Our results show that auxin plays an essential role in EOD-FR-mediated hypocotyl elongation, shed light on the mechanisms of EOD-FR mediated hypocotyl elongation, and provide a theoretical basis for the use of EOD-FR in facility cultivation.

引用

页数：15