Development of an Improved Protocol for the Isolation and Detection of Enterobacter sakazakii (Cronobacter) from Powdered Infant Formula

被引：26

作者：

Chen, Yi ^{[1
]}

Song, Kwang-Yong ^{[1
]}

Brown, Eric W. ^{[1
]}

Lampel, Keith A. ^{[1
]}

机构：

[1] US FDA, College Pk, MD 20740 USA

来源：

JOURNAL OF FOOD PROTECTION | 2010年 / 73卷 / 06期

关键词：

IDENTIFICATION SYSTEMS; CHROMOGENIC MEDIA; SURVIVAL;

D O I：

10.4315/0362-028X-73.6.1016

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Enterobacter sakazakii causes severe maladies and, in some cases, is fatal among infants. Powdered infant formula (PIF) contaminated with E. sakazakii has been documented as a potential cause of several outbreaks involving infants. This study describes the development of a method for the isolation and detection of E. sakazakii from PIP. It combines Taqman real-time PCR, Brilliance E. sakazakii and R&F chromogenic agars, and RAPID ID 32E biochemical tests. This method provides an expedient analysis within I to 2 clays depending on the amount and stress status of E. sakazakii organisms and competing microorganisms in PIF. The real-time PCR has bifunctional applications, including both screening and culture confirmation of E. sakazakii.

引用

页码：1016 / 1022

页数：7

共 50 条

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[2] Method for the isolation and detection of Enterobacter sakazakii (Cronobacter) from powdered infant formula (vol 136, pg 179, 2009)
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