Xclaudin 1 is required for the proper gastrulation in Xenopus laevis

被引:4
|
作者
Chang, Da-Jung [1 ]
Hwang, Yoo-Seok [2 ]
Cha, Sang-Wook [3 ]
Chae, Jeong-Pil [1 ]
Hwang, Sung-Hun [1 ]
Hahn, Jang-Hee [4 ]
Bae, Yong Chul [5 ]
Lee, Hyun-Shik [6 ]
Park, Mae Ja [1 ]
机构
[1] Kyungpook Natl Univ, Sch Med, Dept Anat, Taegu, South Korea
[2] NICHD, Mol Genet Lab, NIH, Bethesda, MD USA
[3] Cincinnati Childrens Res Fdn, Dept Pediat, Div Dev Biol, Cincinnati, OH 45229 USA
[4] Kangwon Natl Univ, Sch Med, Dept Anat & Cell Biol, Chuchon 200701, South Korea
[5] Kyungpook Natl Univ, Sch Dent, Dept Oral Anat, Taegu 700412, South Korea
[6] Kyungpook Natl Univ, Coll Nat Sci, Sch Life Sci, Taegu 702701, South Korea
关键词
Xclaudin; 1; Gastrulation; Convergent extension; Xenopus laevis; TIGHT-JUNCTION PROTEIN; MESENCHYMAL TRANSITION; GENE-EXPRESSION; BREAST-CANCER; CLAUDIN; PATTERN; SNAIL; CELLS;
D O I
10.1016/j.bbrc.2010.05.068
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Claudin I is one of the tight junctional proteins involved in the tight sealing of the cellular sheets and plays a crucial role in the maintenance of cell polarity. Although its structure and physiological function in intercellular adhesion is relatively well understood, we have little information about its possible involvement in early development of vertebrates. We found Xclaudin 1 is expressed maternally in the oocyte of Xenopus laevis and the zygotic expression initiates stage 9 in the animal hemisphere but not in the vegetal hemisphere, limited on the ectoderm and mesoderm until the end of gastrulation. We have investigated a potential role for claudin 1 at gastrulation by gain and loss-of-function studies. Overexpression of Xclaudin 1 resulted in gastrulation defect in a dose-dependent manner. Knockdown of Xclaudin 1 by antisense morpholino oligonucleotides (MOs) blocked convergent extension, whereas ectopic expression of Xclaudin 1-myc mRNA rescued these defects. However, altered expression of Xclaudin 1 did not inhibit mesodermal gene expression. Taken together, our results suggest that Xclaudin 1 is required for proper convergent extension movement during Xenopus gastrulation. (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码:75 / 81
页数:7
相关论文
共 50 条
  • [1] Xenopus glucose transporter 1 (xGLUT1) is required for gastrulation movement in Xenopus laevis
    Suzawa, Keiko
    Yukita, Akira
    Hayata, Tadayoshi
    Goto, Toshiyasu
    Danno, Hiroki
    Michiue, Tatsuo
    Cho, Ken W.
    Asashima, Makoto
    INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY, 2007, 51 (03): : 183 - 190
  • [2] PDGF SIGNALING IS REQUIRED FOR GASTRULATION OF XENOPUS-LAEVIS
    ATALIOTIS, P
    SYMES, K
    CHOU, MM
    HO, L
    MERCOLA, M
    DEVELOPMENT, 1995, 121 (09): : 3099 - 3110
  • [3] Xoom is required for epibolic movement of animal ectodermal cells in Xenopus laevis gastrulation
    Hasegawa, K
    Kinoshita, T
    DEVELOPMENT GROWTH & DIFFERENTIATION, 2000, 42 (04) : 337 - 346
  • [4] A novel gene, BENI is required for the convergent extension during Xenopus laevis gastrulation
    Homma, Motohiro
    Inui, Masafumi
    Fukui, Akimasa
    Michiue, Tatsuo
    Okabayashi, Koji
    Asashima, Makoto
    DEVELOPMENTAL BIOLOGY, 2007, 303 (01) : 270 - 280
  • [5] FoxO genes are dispensable during gastrulation but required for late embryogenesis in Xenopus laevis
    Schuff, Maximilian
    Siegel, Doreen
    Bardine, Nabila
    Oswald, Franz
    Donow, Cornelia
    Knoechel, Walter
    DEVELOPMENTAL BIOLOGY, 2010, 337 (02) : 259 - 273
  • [6] Functional analysis of PTEN at gastrulation of Xenopus laevis
    Kono, Rinco
    Ueno, Shuichi
    Iwao, Yasuhiro
    ZOOLOGICAL SCIENCE, 2004, 21 (12) : 1294 - 1294
  • [7] Functional analysis of PTEN at gastrulation of Xenopus laevis
    Ueno, Shuichi
    Kono, Rinco
    Iwao, Yasuhiro
    ZOOLOGICAL SCIENCE, 2005, 22 (12) : 1458 - 1458
  • [8] Functional analysis of PTEN at gastrulation of Xenopus laevis
    Ueno, S.
    Kono, R.
    Iwao, Y.
    MECHANISMS OF DEVELOPMENT, 2005, 122 : S80 - S80
  • [9] ZFPIP/Zfp462 is maternally required for proper early Xenopus laevis development
    Laurent, Audrey
    Masse, Julie
    Omilli, Francis
    Deschamps, Stephane
    Richard-Parpaillon, Laurent
    Chartrain, Isabelle
    Pellerin, Isabelle
    DEVELOPMENTAL BIOLOGY, 2009, 327 (01) : 169 - 176
  • [10] Myosin-X is required for proper behavior of neural crest cells in Xenopus laevis
    Hwang, Yoo-Seok
    Luo, Ting
    Xu, Yanhua
    Sargent, Thomas D.
    DEVELOPMENTAL BIOLOGY, 2008, 319 (02) : 540 - 540