Haptens, bioconjugates, and antibodies for penthiopyrad immunosensing

被引：6

作者：

Ceballos-Alcantarilla, Eric ^{[2
]}

Abad-Fuentes, Antonio ^{[1
]}

Aloisio, Vincenzo ^{[2
]}

Agullo, Consuelo ^{[2
]}

Abad-Somovilla, Antonio ^{[2
]}

Mercader, Josep V. ^{[1
]}

机构：

[1] CSIC, IATA CSIC, Inst Agrochem & Food Technol, Dept Biotechnol, Valencia 46980, Spain

[2] Univ Valencia, Dept Organ Chem, E-46100 Valencia, Spain

来源：

ANALYST | 2014年 / 139卷 / 21期

关键词：

REGIOISOMERIC HAPTENS; GENERATION; RESISTANCE;

D O I：

10.1039/c4an00828f

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Haptens, bioconjugates, and antibodies for highly sensitive immunochemical analysis of the new-generation fungicide penthiopyrad are described. Two haptens with equivalent carboxylated linkers were prepared, and the purified active esters were efficiently coupled to proteins. The results revealed slightly different antibody-eliciting capacities for the two synthetic derivatives. All of the produced antibodies were specific for penthiopyrad, and showed affinity values in the nanomolar range.

引用

页码：5358 / 5361

页数：4

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