A bifunctional fluorescent probe for simultaneous detection of GSH and H2Sn (n > 1) from different channels with long-wavelength emission

被引：8
|
作者：
Niu, Peixin [1 ]
Rong, Yifan [1 ]
Wang, Yuyue [1 ]
Ni, Huijie [1 ]
Zhu, Minghui [1 ]
Chen, Wenqiang [3 ]
Liu, Xingjiang [1 ]
Wei, Liuhe [1 ]
Song, Xiangzhi [2 ]
机构：
[1] Zhengzhou Univ, Coll Chem, Green Catalysis Ctr, Zhengzhou 450001, Henan, Peoples R China
[2] Cent South Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China
[3] Nanning Normal Univ, Guangxi Key Lab Nat Polymer Chem & Phys, Nanning 530001, Guangxi, Peoples R China
来源：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2021年 / 257卷
基金：
中国国家自然科学基金;
关键词：
Glutathione; Polysulfide; Long-wavelength emission; Cell imaging; Fluorescent probe; HYDROGEN-SULFIDE; CYSTEINE PERSULFIDES; OXIDATIVE STRESS; NITRIC-OXIDE; LIVING CELLS; ON PROBE; GLUTATHIONE; POLYSULFIDES; PEROXIDE; BIOLOGY;
D O I：
10.1016/j.saa.2021.119789
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
In this work, we presented a long-wavelength emission fluorescent probe DCM-Cou-SePh that can discriminatively detect glutathione (GSH) and hydrogen polysulfides (H2Sn, n > 1) from green and red emission channels, respectively. With the addition of GSH, probe DCM-Cou-SePh displayed green fluorescence emission (lambda(ex/em) = 430/530 nm). In the presence of H2Sn, the probe exhibited a significant fluorescence enhancement in red channel (lambda(ex/em) = 560/680 nm). We also demonstrated that this probe was suitable to quantitatively detect GSH and H2Sn with low detection limits (0.12 mu M for GSH, 0.19 mu M for H2Sn). Furthermore, DCM-Cou-SePh can be used for sensing endogenous GSH and H2Sn in living cells by dual-color fluorescence imaging. (C) 2021 Elsevier B.V. All rights reserved.
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页数：8
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