Culture phases, cytotoxicity and protein expressions of agarose hydrogel induced Sp2/0, A549, MCF-7 cell line 3D cultures

被引：14

作者：

Ravi, Maddaly ^{[1
]}

Kaviya, S. R. ^{[1
]}

Paramesh, V. ^{[1
]}

机构：

[1] Sri Ramachandra Univ, Fac Biomed Sci Technol & Res, Dept Human Genet, Chennai 600116, Tamil Nadu, India

来源：

CYTOTECHNOLOGY | 2016年 / 68卷 / 03期

关键词：

Agarose hydrogels; 3D aggregates; Cell culture phases; Cytotoxicity; Protein expressions; HUMAN CANCER; ADHESION; MASPIN; 2D;

D O I：

10.1007/s10616-014-9795-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Advancements in cell cultures are occurring at a rapid pace, an important direction is culturing cells in 3D conditions. We demonstrate the usefulness of agarose hydrogels in obtaining 3 dimensional aggregates of three cell lines, A549, MCF-7 and Sp2/0. The differences in culture phases, susceptibility to cisplatin-induced cytotoxicity are studied. Also, the 3D aggregates of the three cell lines were reverted into 2D cultures and the protein profile differences among the 2D, 3D and revert cultures were studied. The analysis of protein profile differences using UniProt data base further augment the usefulness of agarose hydrogels for obtaining 3D cell cultures.

引用

页码：429 / 441

页数：13

共 13 条

[1] Culture phases, cytotoxicity and protein expressions of agarose hydrogel induced Sp2/0, A549, MCF-7 cell line 3D cultures
Maddaly Ravi
S. R. Kaviya
V. Paramesh
Cytotechnology, 2016, 68 : 429 - 441
[2] Agarose hydrogel induced MCF-7 and BMG-1 cell line progressive 3D and 3D revert cultures
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[3] Significance of cell culture phases for utilizing 3D cultures of A549 cells for in vitro studies
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[4] Cytokine profiling of MCF-7 cell line 2D, progressive 3D, and 3D revert cultures
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BIOTECHNOLOGY JOURNAL, 2019, 14 (04)
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