Tellurite and Tellurate Reduction by the Aerobic Anoxygenic Phototroph Erythromonas ursincola, Strain KR99 Is Carried out by a Novel Membrane Associated Enzyme

被引：18

作者：

Maltman, Chris ^{[1
]}

Donald, Lynda J. ^{[1
]}

Yurkov, Vladimir ^{[1
]}

机构：

[1] Univ Manitoba, Dept Microbiol, Winnipeg, MB R3T 2N2, Canada

来源：

MICROORGANISMS | 2017年 / 5卷 / 02期

基金：

加拿大自然科学与工程研究理事会;

关键词：

tellurite; tellurate; tellurium; reduction; aerobic anoxygenic phototroph; JOAQUIN DRAINAGE WATER; BIOCHEMICAL-CHARACTERIZATION; REDUCING ACTIVITIES; THAUERA-SELENATIS; SENSITIVE METHOD; SP-NOV; RESISTANCE; BACTERIA; PROTEINS; SELENITE;

D O I：

10.3390/microorganisms5020020

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Erythromonas ursincola, strain KR99 isolated from a freshwater thermal spring of Kamchatka Island in Russia, resists and reduces very high levels of toxic tellurite under aerobic conditions. Reduction is carried out by a constitutively expressed membrane associated enzyme, which was purified and characterized. The tellurite reductase has a molecular weight of 117 kDa, and is comprised of two subunits (62 and 55 kDa) in a 1:1 ratio. Optimal activity occurs at pH 7.0 and 28 degrees C. Tellurite reduction has a V-max of 5.15 mu mol/min/mg protein and a K-m of 3.36 mM. The enzyme can also reduce tellurate with a V-max and K-m of 1.08 mu mol/min/mg protein and 1.44 mM, respectively. This is the first purified membrane associated Te oxyanion reductase.

引用

页数：8