Main Analytical Methods for the Viability Assessment of Mesenchymal Stem Cells for Use as Cellular Medicines

被引：4
|
作者：
Galocha-Leon, Cristina [1 ]
Clares-Naveros, Beatriz [1 ]
Galvez-Martin, Patricia [1 ,2 ]
机构：
[1] Univ Granada, Sch Pharm, Dept Pharm & Pharmaceut Technol, E-18071 Granada, Spain
[2] Bioiberica SAU, Adv Therapies Area, E-08029 Barcelona, Spain
来源：
CURRENT PHARMACEUTICAL ANALYSIS | 2018年 / 14卷 / 05期
关键词：
Mesenchymal stem cells; cell therapy; cell viability; cell count; analytical methods; quality control assays; FLUORESCENT-PROTEIN BIOSENSORS; TRYPAN BLUE; CYTOTOXICITY; GROWTH; SYSTEM; ASSAYS; TOOLS;
D O I：
10.2174/1573412913666170613090734
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Background: Mesenchymal Stem Cells (MSCs) are multipotent stem cells with therapeutic potential in advanced therapies and regenerative medicine. Currently, MSCs are being widely studied as cellular medicine. In the design and development of medicines with cells, the study of viability is an important quality control requirement. Objective: Description of the main analytical techniques to study the MSCs viability as cellular medicine. Results: The cellular medicine must contain viable cells, for its use in vivo the cell viability must be at least greater or equal to 80%. Therefore, the study of cell viability of MSCs during its ex vivo expansion and its final formulation is a prerequisite before being administered. Conclusion: In this review the main analytical techniques based on studies of the cell membrane integrity, morphological assays and fluorescence biosensors are described. All of them are approved for use in quality control programs for clinical use of MSCs.
引用
收藏
页码：427 / 436
页数：10
相关论文
共 50 条
  • [1] In vitro augmentation of mesenchymal stem cells viability in stressful microenvironmentsIn vitro augmentation of mesenchymal stem cells viability
    Fatemeh Amiri
    Ali Jahanian-Najafabadi
    Mehryar Habibi Roudkenar
    Cell Stress and Chaperones, 2015, 20 : 237 - 251
  • [2] Viability of mesenchymal stem cells during electrospinning
    Zanatta, G.
    Steffens, D.
    Braghirolli, D. I.
    Fernandes, R. A.
    Netto, C. A.
    Pranke, P.
    BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH, 2012, 45 (02) : 125 - 130
  • [3] Mesenchymal stem cells as cellular immunosuppressants
    Wolf, Dominik
    Wolf, Anna Maria
    LANCET, 2008, 371 (9624): : 1553 - 1554
  • [4] Surfactant effects on the viability and function of human mesenchymal stem cells: in vitro and in vivo assessment
    Chung-Ming Chen
    Hsiu-Chu Chou
    Willie Lin
    Chris Tseng
    Stem Cell Research & Therapy, 8
  • [5] Surfactant effects on the viability and function of human mesenchymal stem cells: in vitro and in vivo assessment
    Chen, Chung-Ming
    Chou, Hsiu-Chu
    Lin, Willie
    Tseng, Chris
    STEM CELL RESEARCH & THERAPY, 2017, 8
  • [6] Use of Adipose-Derived Mesenchymal Stem Cells to Increase Viability of Composite Grafts
    Yucel, Ergin
    Alagoz, Murat Sahin
    Eren, Guler Gamze
    Yasar, Emrah Kagan
    Izmirli, Hakki Hayrettin
    Duruksu, Gokhan
    Isgoren, Serkan
    Muezzinoglu, Bahar
    Karaoz, Erdal
    JOURNAL OF CRANIOFACIAL SURGERY, 2016, 27 (05) : 1354 - 1360
  • [7] The effect of temperature on the viability of human mesenchymal stem cells
    Yannis Reissis
    Elena García-Gareta
    Michelle Korda
    Gordon W Blunn
    Jia Hua
    Stem Cell Research & Therapy, 4
  • [8] POST-CRYOPRESERVATION VIABILITY OF MESENCHYMAL STEM CELLS
    Van Campenhout, A.
    Swinnen, L.
    Klykens, J.
    Devos, T.
    Verhoef, G.
    CYTOTHERAPY, 2014, 16 (04) : S83 - S83
  • [9] The effect of temperature on the viability of human mesenchymal stem cells
    Reissis, Yannis
    Garcia-Gareta, Elena
    Korda, Michelle
    Blunn, Gordon W.
    Hua, Jia
    STEM CELL RESEARCH & THERAPY, 2013, 4
  • [10] COMPARISON OF TWO AUTOMATED CELL COUNTERS FOR ENUMERATION AND VIABILITY OF MESENCHYMAL STEM CELLS FOR CLINICAL CELLULAR THERAPY TRIALS
    Radel, D. J.
    Madde, P.
    Dietz, A.
    CYTOTHERAPY, 2018, 20 (05) : S69 - S70
12345