Macromolecular transport in heart valves. III. Experiment and theory for the size distribution of extracellular liposomes in hyperlipidemic rabbits

The heart valve leaflets of 29-day cholesterol-fed rabbits were examined by ultrarapid freezing, without conventional chemical fixation/processing, followed by rotary shadow freeze-etching,. This procedure images the leaflets' subendothelial extracellular matrix in extraordinary detail, and extracellular lipid liposomes. from 23 to 220 nm in diameter, clearly appear there. These liposomes are linked to matrix filaments and appear ill Clusters. Their size distribution shows 60.7% with diameters 23-69 nm. 31.7% between 70 and 119 nm 7.3% between 120 and 169 nm and 0.3% between 170 and 220 run (superlarge) and suggests that smaller liposomes can fuse into larger ones. We Couple our model front Part II of this series (Zeng Z. Yin Y, Jan KM, Rumschitzki DS. Am J Physiol Heart Circ Physiol 292: H2671-H2686, 2007) for lipid transport into the leaflet to the nucleation-polymerization model hierarchy for liposome formation proposed originally for aortic liposomes to predict liposome formation/growth in heart valves. simulations show that the simplest Such model cannot account for the observed size distribution. However, modifying this model by including liposome fusing/merging, using parameters determined from aortic liposomes. leads to predicted size distributions in excellent agreement with our valve data. Evolutions of both the liposome size distribution and total liposome mass suggest that fusing becomes significant only after 2 wk of high lumen cholesterol. Inclusion of phagocytosis by macrophages limits the otherwise monotonically increasing total liposome mass. while keeping the excellent fit of the liposome size distribution to the data.