Effect of iron-sulfur cluster assembly proteins on the expression of Escherichia coli lipoic acid synthase

被引：30
|
作者：
Kriek, M
Peters, L
Takahashi, Y
Roach, PL [1 ]
机构：
[1] Univ Southampton, Dept Chem, Southampton SO17 1BJ, Hants, England
[2] Osaka Univ, Grad Sch Sci, Dept Biol, Osaka 5600043, Japan
来源：
PROTEIN EXPRESSION AND PURIFICATION | 2003年 / 28卷 / 02期
关键词：
D O I：
10.1016/S1046-5928(02)00680-0
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Lipoic Acid Synthase (LipA) can accommodate a [4Fe-4S] cluster that is thought to be essential for the insertion of sulfur into an octanoyl substrate during the biosynthesis of lipoic acid. With the objective of improving soluble holo-LipA expression, a series of multi-cistronic plasmids were constructed carrying lipA in combination with one of the three systems: groE/SL, trxA, or the isc operon. Co-expression of lipA with the isc operon approximately trebled the isolated yield of soluble LipA and resulted in efficient assembly of the Fe-S cluster. This strategy may be helpful in the soluble expression of a wide range of Fe-S cluster-dependent proteins. (C) 2002 Elsevier Science (USA). All rights reserved.
引用
收藏
页码：241 / 245
页数：5
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