Dynamics of memory and naive CD8+ T lymphocytes in humanized NOD/SCID/IL-2Rγnull mice infected with CCR5-tropic HIV-1

被引：37
|
作者：
Sato, Kei [1 ]
Nie, Chuanyi [1 ]
Misawa, Naoko [1 ]
Tanaka, Yuetsu [2 ]
Ito, Mamoru [3 ]
Koyanagi, Yoshio [1 ]
机构：
[1] Kyoto Univ, Inst Virus Res, Lab Viral Pathogenesis, Sakyo Ku, Kyoto 6068507, Japan
[2] Univ Ryukyus, Grad Sch Med, Dept Immunol, Okinawa 9030125, Japan
[3] Cent Inst Expt Anim, Kawasaki Ku, Kanagawa 2160001, Japan
来源：
VACCINE | 2010年 / 28卷
基金：
日本学术振兴会;
关键词：
HIV-1; Humanized mice; lmmunopathogenesis; HUMAN-IMMUNODEFICIENCY-VIRUS; SCID-HU MOUSE; HUMORAL IMMUNE-RESPONSES; HEMATOPOIETIC STEM-CELLS; CENTRAL-NERVOUS-SYSTEM; BLOOD CD34(+) CELLS; EPSTEIN-BARR-VIRUS; CORD BLOOD; IN-VIVO; MODEL;
D O I：
10.1016/j.vaccine.2009.10.154
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Creating a novel small animal model of HIV-1 infection that can support long-term systemic HIV-1 infection and produce HIV-1-specific immune response has a great benefit for studying HIV-1 pathogenesis in vivo. In the present study, we have generated a humanized mouse, NOG-hCD34 mouse, by transplanting newborn NOD/SCID/IL-2R gamma(null) mice with human hematopoietic stem cells through hepatic injection. These mice were infected with a CCR5-tropic HIV-1 and were analyzed for plasma viral load, changes in peripheral blood T lymphocytes, and HIV-1-specific antibody production. High level of viral replication, increase in effector/memory CD8(+) T lymphocytes, class-switching to IgG, and production of HIV-1-specific IgGs were observed. Our findings suggest that NOG-hCD34 mice may have a wide variety of application in HIV-1 research. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码：B32 / B37
页数：6
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