PI/DAPI Fluorochrome Staining Technique for Identifying Citrus Chromosomes

被引：0
|
作者：
Yu Yang [1 ]
Wang Wei [1 ]
Yang Xiao-ling [1 ]
机构：
[1] Tongji Univ, Coll Life Sci & Technol, Shanghai 200092, Peoples R China
来源：
2009 ACADEMIC CONFERENCE ON HORTICULTURE SCIENCE AND TECHNOLOGY, PROCEEDINGS | 2009年
关键词：
PI/DAPI staining; chromosome; citrus; karyotype; CMA BANDING-PATTERNS; IDENTIFICATION; VARIABILITY; PONCIRUS;
D O I：
暂无
中图分类号：
S6 [园艺];
学科分类号：
0902 ;
摘要：
The mitotic metaphase chromosomes of several citrus were stained with propidium iodide (PI) and (DAPI). The stable chromosome patterns were observed with the suitable proportion of PI/DAPI volume. Based on the relative-sized PI signals and the morphology of no-signal chromosomes, 18 chromosomes of citrus were classified into 7 groups. In addition, the relative-sized PI signals on the chromosomes were observed maintain stable in the cell cycle. It is considered that the chromosome combined with PI fluorochrome were highly stable construction.
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页码：53 / 55
页数：3
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