Effect of a novel water-soluble vitamin E derivative, 2-(α-D-glucopyranosyl)methyl-2,5,7,8-tetramethylchroman-6-ol, on dextran sulfate sodium-induced colitis in mice

Oxygen radical-mediated lipid peroxidation is involved in the tissue injury of inflammatory bowel disease. The present study investigated the effects of a novel water-soluble vitamin E derivative, 2-(a-D-glucopyranosyl)methyl-2,5,7,8-tetramethylchroman-6-ol (TMG), on dextran sulfate sodium-induced colonic inflammation in mice. Acute colitis was induced in female mice by giving 8% dextran sulfate sodium orally in drinking water for 7 days. Animals were randomized to different concentrations of TMG (1, 10, and 100 mg/kg) or physiologic saline (vehicle) by daily intraperitoneal injection. After 7 days of dextran sulfate sodium. administration, mice had severe colonic inflammation characterized by significant decreases in total colon length, increases in luminal hemoglobin content and colonic myeloperoxidase activity. TMG at doses of 10 and 100 mg/kg reduced colonic injury and inflammation. The contents of thiobarbituric acid-reactive substances were significantly increased by dextran sulfate sodium administration, and this increase was reduced by TMG. These results indicate that the protective effect of TMG against colonic injury induced by dextran sulfate sodium may result, in part, from its inhibitory action toward lipid peroxidation, as well as from reduced neutrophil recruitment into the inflammatory site.