GLUT-3 expression in human skeletal muscle

被引:18
|
作者
Stuart, CA
Wen, G
Peng, BH
Popov, VL
Hudnall, SD
Campbell, GA
机构
[1] Univ Texas, Med Branch, Dept Internal Med, Galveston, TX 77555 USA
[2] Univ Texas, Med Branch, Dept Pathol, Galveston, TX 77555 USA
关键词
GLUT-3; human muscle; immunofluorescence; in situ hybridization;
D O I
10.1152/ajpendo.2000.279.4.E855
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Muscle biopsy homogenates contain GLUT-3 mRNA and protein. Before these studies, it was unclear where GLUT-3 was located in muscle tissue. In situ hybridization using a midmolecule probe demonstrated GLUT-3 within all muscle fibers. Fluorescent-tagged antibody reacting with affinity-purified antibody directed at the carboxy-terminus demonstrated GLUT-3 protein in all fibers. Slow-twitch muscle fibers, identified by NADH-tetrazolium reductase staining, possessed more GLUT-3 protein than fast-twitch fibers. Electron microscopy using affinity-purified primary antibody and gold particle-tagged second antibody showed that the majority of GLUT-3 was in association with triads and transverse tubules inside the fiber. Strong GLUT-3 signals were seen in association with the few nerves that traversed muscle sections. Electron microscopic evaluation of human peripheral nerve demonstrated GLUT-3 within the axon, with many of the particles related to mitochondria. GLUT-3 protein was found in myelin but not in Schwann cells. GLUT-1 protein was not present in nerve cells, axons, myelin, or Schwann cells but was seen at the surface of the peripheral nerve in the perineurium. These studies demonstrated that GLUT-3 mRNA and protein are expressed throughout normal human skeletal muscle, but the protein is predominantly found in the triads of slow-twitch muscle fibers.
引用
收藏
页码:E855 / E861
页数:7
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