Best practices and tools for reporting reproducible fluorescence microscopy methods

被引：68

作者：

Llopis, Paula Montero ^{[1
]}

Senft, Rebecca A. ^{[2
]}

Ross-Elliott, Tim J. ^{[1
]}

Stephansky, Ryan ^{[1
]}

Keeley, Daniel P. ^{[3
]}

Koshar, Preman ^{[3
]}

Marques, Guillermo ^{[4
,5
]}

Gao, Ya-Sheng ^{[6
]}

Carlson, Benjamin R. ^{[6
]}

Pengo, Thomas ^{[7
]}

Sanders, Mark A. ^{[4
,5
]}

Cameron, Lisa A. ^{[6
]}

Itano, Michelle S. ^{[3
]}

机构：

[1] Harvard Med Sch, MicRoN Core, Boston, MA 02115 USA

[2] Harvard Med Sch, Blavatnik Inst, Dept Genet, Boston, MA 02115 USA

[3] Univ N Carolina, Neurosci Microscopy Core, Chapel Hill, NC 27515 USA

[4] Univ Minnesota, Univ Imaging Ctr, Minneapolis, MN USA

[5] Univ Minnesota, Dept Neurosci, Minneapolis, MN USA

[6] Duke Univ, Duke Light Microscopy Core Facil, Durham, NC USA

[7] Univ Minnesota, Informat Inst, Minneapolis, MN USA

来源：

NATURE METHODS | 2021年 / 18卷 / 12期

基金：

美国国家卫生研究院;

关键词：

OXYGEN SPECIES PRODUCTION; BEGINNERS GUIDE; CELL; PHOTOTOXICITY; PROTEINS; QUALITY; PERFORMANCE; RESOLUTION; ACCURACY;

D O I：

10.1038/s41592-021-01156-w

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Comprehensive guidelines and resources to enable accurate reporting for the most common fluorescence light microscopy modalities are reported with the goal of improving microscopy reporting, rigor and reproducibility. Although fluorescence microscopy is ubiquitous in biomedical research, microscopy methods reporting is inconsistent and perhaps undervalued. We emphasize the importance of appropriate microscopy methods reporting and seek to educate researchers about how microscopy metadata impact data interpretation. We provide comprehensive guidelines and resources to enable accurate reporting for the most common fluorescence light microscopy modalities. We aim to improve microscopy reporting, thus improving the quality, rigor and reproducibility of image-based science.

引用

页码：1463 / +

页数：17

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