The Promoter Hypermethylation of DAPK Gene and p16 Gene in Non-small Cell Lung Cancer Patients

Objective: To evaluate the clinical significan cc of the aberrant methylation of DAPK gene and p16 gene in serum from 65 NSCLC patients from the first affiliated hospital of suzhou university. Mothods : A methylation-specific PCR (MSP)was performed for the detection of promoter hypermethylation of DAPK gene and p16 gene in blood DNA from 65 cases of NSCLC. and to analyze the relation of the aberrant methylation of DAPK gene and p16 gene and the clinicopathological data. Results: 30.8% (20 / 65)of the sera from 65 cases of NSCLC showed hypermethylation for DAPK promoter and 43.1 % (28 / 65)the same for p16 promoter, whereas no methylated DAPK gene promoter and p16 gene promoter were found in sera from the patients with lung benign diseases and normal controls. Methylated DAPK gene promoter and p16 gene promoter in sera were not closely correlated with the pathological classification, stage, metastas is and differentiation in NSCLC. Conclusion Detection of the aberrant methylation of DAPK gene and p 16 gene in blood DNA from NSCLC patients might offer an effective meaa-1S for the earlier auxiliar y diagnosis of the malignancy.