Comprehensive Identification of Short and Medium-Sized Peptides from Pixian Broad Bean Paste Protein Hydrolysates Using UPLC-Q- TOF-MS and UHPLC-Q Exactive HF-X

被引：20

作者：

Li, Mingyang ^{[1
,2
]}

Fan, Wenlai ^{[1
,2
]}

Xu, Yan ^{[1
,2
]}

机构：

[1] Jiangnan Univ, Sch Biotechnol, Lab Brewing Microbiol & Appl Enzymol, Wuxi 214122, Jiangsu, Peoples R China

[2] Jiangnan Univ, Sch Biotechnol, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Jiangsu, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2022年 / 70卷 / 27期

基金：

国家重点研发计划;

关键词：

Pixian broad bean paste (PBBP); enzymatic protein hydrolysis; short peptides; medium-sized peptides; peptidomics; ACE-INHIBITORY PEPTIDES; ANTIOXIDANT PEPTIDES; BIOACTIVE PEPTIDES; ENZYME; QUADRUPOLE; SYSTEM;

D O I：

10.1021/acs.jafc.2c02487

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Pixian broad bean paste (PBBP) is an indispensable food widely used in many East Asian countries, yet the knowledge about bioactive peptides released from parent proteins by enzymatic hydrolysis is limited. A total of 4867 low-molecular weight peptides were identified in the highly bioactive subfractions of the PBBP alcalase hydrolysates using traditional and peptidomics approaches. 19 short peptides (3-5 amino acids) were identified by ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry, including 5 tripeptides, 8 tetrapeptides, and 6 pentapeptides. 5848 medium-sized peptides (6-10 amino acids) were characterized using the peptidomics approach, including 1484 hexapeptides, 1217 heptapeptides, 1634 octapeptides, 927 nonapeptides, and 586 decapeptides. The comprehensive method can be used for the investigation of bioactive peptides in complex food matrices.

引用

页码：8288 / 8299

页数：12