Production of DNA-hydrolyzing antibody BV04-01 Fab fragment in methylotrophic yeast Pichia pastoris

被引：3
|
作者：
Kozyr, AV [1 ]
Bobik, TV [1 ]
Ignatova, AN [1 ]
Kolesnikov, AV [1 ]
机构：
[1] Russian Acad Sci, Shemyakin & Ovchinnikov Inst Biooorgan Chem, Moscow 117997, Russia
来源：
MOLECULAR BIOLOGY | 2004年 / 38卷 / 06期
基金：
俄罗斯基础研究基金会;
关键词：
recombinant antibody production; Fab fragment; Pichia pastoris;
D O I：
10.1023/B:MBIL.0000049871.17260.46
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A system was developed for efficient production of recombinant Fab of catalytic DNA-hydrolyzing antibody BV04-01 in methylotrophic yeast Pichia pastoris. To stabilize Fab, the C ends of its chains were modified with dimerizing Jun and Fos, which are known to form a leucine zipper. The yield of functional recombinant Fab was 3 mg per liter culture. The catalytic efficiency of Fab was 1.8 (.) 10(6) M-1 min(-1) as inferred from the relaxation of supercoiled plasmid DNA.
引用
收藏
页码：914 / 920
页数：7
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