Protein partitioning in aqueous two-phase systems formed by mixtures of polymers and cationic-anionic surfactants

被引：0

作者：

Xiao, JX ^{[1
]}

Huang, JB ^{[1
]}

He, X ^{[1
]}

Bao, YX ^{[1
]}

Zhu, BY ^{[1
]}

机构：

[1] Peking Univ, Coll Chem & Mol Engn, Lab Colloid Chem, Beijing 100871, Peoples R China

来源：

ACTA CHIMICA SINICA | 2000年 / 58卷 / 07期

关键词：

aqueous two-phases; protein partitioning; random copolymers; cationic-anionic surfactants;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel aqueous two - phase system (ATPS) can be formed by mixing a polymer and a cationic anionic surfactant. In this work we investigated the protein partitioning in ATPS formed by mixing dodecyltriethylammonium bromide/sodium dodecylsulfate and ethylene oxide (EO) - propylene oxide (PO) random copolymers (EO20PO80). Affinity ligands were attached to polymers and affinity partitioning of proteins was investigated. A multi - step partitioning procedure was developed by diluting the surfactant - enriched phase or by raising the temperature of polymer - enriched phase. After partitioning was completed, the surfactants could be removed when the phases were further diluted, and EO - PO copolymers could be separated by raising the temperature above the cloud point of copolymer. The recycling of cationic - anionic surfactants and EO - PO copolymers is discussed.

引用

页码：922 / 924

页数：3

共 4 条

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[4] Aqueous two-phase systems of the aqueous mixtures of cationic anionic surfactants
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