Enhanced biosynthesis of triterpenoids in Centella asiatica hairy root culture by precursor feeding and elicitation

被引:31
|
作者
Baek, Seungeun [1 ,2 ]
Thanh-Tam Ho [1 ]
Lee, Hyoshin [3 ]
Jung, Gooyoung [4 ]
Kim, Young Eun [1 ,4 ]
Jeong, Cheol-Seung [1 ,5 ]
Park, So-Young [1 ]
机构
[1] Chungbuk Natl Univ, Div Anim Hort & Food Sci, Dept Hort Sci, Cheongju 28644, South Korea
[2] Hankook Cosmet Mfg, 20F,35 Cheonggyecheon Ro, Seoul, South Korea
[3] Natl Inst Forest Sci, Dept Forest Genet Resources, 39 Onjeong Ro, Suwon 16631, South Korea
[4] Dongkook Pharmaceut Co Ltd, 1103 Jingwang Ro, Jincheon Gun, Chungcheongbuk, South Korea
[5] WellGreen Co Ltd, Chungdae Ro 1, Cheongju 28644, South Korea
关键词
Asiaticoside; Centella asiatica; Elicitation; Hairy root culture; Precursor feeding; Triterpenoid; CELL-SUSPENSION CULTURES; METHYL JASMONATE; GINSENOSIDE ACCUMULATION; SECONDARY METABOLISM; MEDICINAL HERB; BETA-VULGARIS; L; URBAN; ELICITORS; COMBINATION; GROWTH;
D O I
10.1007/s11816-019-00573-w
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Centella asiatica contains various triterpenoids, including madecassoside, asiaticoside, madecassic acid, and asiatic acid. Among them, they are mainly contained in leaf, and among them asiaticoside is known as tissue-specific compound that are mainly accumulated in the leaves. Therefore, in the present study, C. asiatica hairy root cultures were established from petiole and leaf explants using Agrobacterium rhizogenes-mediated transformation. To promote the biosynthesis of these triterpenoids, hairy root cultures were treated with precursors (squalene and pyruvic acid) and elicitors [methyl jasmonate (MeJA) and salicylic acid (SA)] at various concentrations. No significant difference was detected in the growth index of hairy root cultures between precursor feeding and elicitation, except pyruvic acid feeding, suggesting that elicitors do not affect the growth of hairy roots. Treatment with 2.5 mM squalene and 5 mM pyruvic acid enhanced the biosynthesis of target triterpenoids (57.53 and 29.13 mg g(-1) DW, respectively) compared with other treatments. However, compared to squalene, MeJA showed a greater effect on the triterpene saponin content in C. asiatica. The highest quantity of triterpenoids (60.25 mg g(-1) DW) was produced in hairy root cultures treated with 400 mu M MeJA. These results indicate that precursor- and elicitor-treated C. asiatica hairy root cultures rapidly accumulate biomass and could be used to enhance the production of triterpenoids for industrial purposes.
引用
收藏
页码:45 / 53
页数:9
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