Effect of Nitric Oxide on the Functioning of the P-Glycoprotein Transporter

被引：1

作者：

Abalenikhina, Yu, V ^{[1
]}

Sudakova, E. A. ^{[1
]}

Slepnev, A. A. ^{[1
]}

Shchul'kin, A., V ^{[1
]}

Yakusheva, E. N. ^{[1
]}

机构：

[1] Ryazan State Med Univ, Minist Hlth Russian Federat, Ryazan, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2022年 / 173卷 / 01期

关键词：

P-glycoprotein; ABCB1-protein; nitric oxide II (NO); S-nitrosoglutathione; EXPRESSION; NO;

D O I：

10.1007/s10517-022-05486-3

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

We studied the effect of nitric oxide (NO) on the functioning of P-glycoprotein transporter (Pgp) in Caco-2 cells. NO donor S-nitrosoglutathione (GSNO) was used in concentrations of 1, 10, 50, 100, and 500 mu M; the duration of exposure was 24 h. The content of Pgp was analyzed by the Western blotting, activity of the transport protein was analyzed by the transport of its substrate fexofenadine. It was shown that GSNO in concentrations of 10 and 50 mu M increased the content and activity of Pgp. Increasing the GSNO concentration to 500 mu M led to the development of nitrosative stress and a decrease in the content and activity of the transporter protein.

引用

页码：28 / 32

页数：5

共 50 条

[1] Effect of Nitric Oxide on the Functioning of the P-Glycoprotein Transporter
Yu. V. Abalenikhina
E. A. Sudakova
A. A. Slepnev
A. V. Shchul’kin
E. N. Yakusheva
Bulletin of Experimental Biology and Medicine, 2022, 173 : 28 - 32
[2] Mechanisms of Regulation of the P-Glycoprotein Transporter Protein Functioning under the Action of Nitric Oxide
Shchulkin, Aleksey, V
Abalenikhina, Yulia, V
Sudakova, Elena A.
Mylnikov, Pavel Yu
Yakusheva, Elena N.
BIOCHEMISTRY-MOSCOW, 2022, 87 (04) : 366 - 379
[3] Mechanisms of Regulation of the P-Glycoprotein Transporter Protein Functioning under the Action of Nitric Oxide
Aleksey V. Shchulkin
Yulia V. Abalenikhina
Elena A. Sudakova
Pavel Yu. Mylnikov
Elena N. Yakusheva
Biochemistry (Moscow), 2022, 87 : 366 - 379
[4] The P-glycoprotein multidrug transporter
Sharom, Frances J.
ESSAYS IN BIOCHEMISTRY: ABC TRANSPORTERS, 2011, 50 : 161 - 178
[5] The P-glycoprotein multidrug transporter
Fardel, O
Lecureur, V
Guillouzo, A
GENERAL PHARMACOLOGY, 1996, 27 (08): : 1283 - 1291
[6] P-GLYCOPROTEIN TRANSPORTER IN DRUG DEVELOPMENT
Prachayasittikul, Veda
Prachayasittikul, Virapong
EXCLI JOURNAL, 2016, 15 : 113 - 118
[7] Kinetics of the P-glycoprotein, the multidrug transporter
Stein, WD
EXPERIMENTAL PHYSIOLOGY, 1998, 83 (02) : 221 - 232
[8] The effect of macrolide antibiotics on the function of the drug transporter P-glycoprotein
Eberl, S
Bachmakov, I
Dörje, F
Fromm, MF
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 2005, 371 : R145 - R145
[9] Effect of P-glycoprotein modulators on the human extraneuronal monoamine transporter
Martel, F
Keating, E
Azevedo, I
EUROPEAN JOURNAL OF PHARMACOLOGY, 2001, 422 (1-3) : 31 - 37
[10] Nitric oxide and P-glycoprotein modulate the phagocytosis of colon cancer cells
Kopecka, Joanna
Campia, Ivana
Brusa, Davide
Doublier, Sophie
Matera, Lina
Ghigo, Dario
Bosia, Amalia
Riganti, Chiara
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2011, 15 (07) : 1492 - 1504

← 1 2 3 4 5 →