EFFECT OF ETO AND HYDROGEN PEROXIDE PLASMA STERILISATION METHODS ON CELL PROLIFERATION ABILITY IN PLLA/GA SCAFFOLDS

The purpose of the research was to discuss if there is any difference between the effects of two low-temperature sterilisation methods: ETO (ethylene oxide) and hydrogen peroxide plasma to cell proliferation abilities in L-polylactic acid/gelatin scaffold materials. By applying MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) experiments and SEM (scanning electron microscope), we can make a comparison between these two sterilisation methods in BMSCs (bone marrow mesenchymal stem cells) proliferation on scaffolds at 4 timings: 2, 4, 6 and 8 days. SEM suggests that both scaffolds are suitable for cell growth and cells are in good shape; MTT suggests that OD (optical density) value of hydrogen peroxide plasma group is higher than that of ETO group in 4, 6 and 8 days indicating that the cell proliferation ability is strong. It was concluded that both those two sterilisation methods can be applied to the sterilisation of cell proliferation scaffolds and hydrogen peroxide plasma can make the cell grow better.