Nonomuraea ceibae sp nov., an actinobacterium isolated from Ceiba speciosa rhizosphere

Strain XMU 110(T), isolated from the rhizosphere soil of a flowering tree, Ceiba speciosa, was characterized by polyphasic taxonomy. Phylogenetic analysis based on 16S rRNA gene comparisons revealed that strain XMU 110(T) showed the highest similarity of 97.9% to Nonomuraea jabiensis DSM 45507(T), and indicated the closest relatives were Nonomuraea roseoviolacea subsp. roseoviolacea ATCC 27297(T) (97.8% 16S rRNA gene sequence similarity) and Nonomuraea salmonea DSM 43678(T) (97.4 %) after a neighbour-joining analysis. The phenotypic characteristics, as well as the DNA-DNA relatedness values between strain XMU 110(T) and N. roseoviolacea subsp. roseoviolacea ATCC 27297(T) ( 48.07 +/- 1.99 %) and N. salmonea DSM 43678(T) ( 40.55 +/- 8.30 %), distinguished the novel strain from its closest phylogenetic neighbours. The morphological, physiological and chemotaxonomic characteristics such as phospholipid type, diagnostic diamino acid of the peptidoglycan, whole-cell sugars, major menaquinones and major fatty acids further supported the assignment of strain XMU 110(T) to the genus Nonomuraea. The G+C content of the genomic DNA was 66.2 mol%. Based on the taxonomic data, strain XMU 110(T) represents a novel species of the genus Nonomuraea, for which the name Nonomuraea ceibae sp. nov. is proposed. The type strain is XMU 110(T) (= MCCC 1K03213(T) = KCTC 39826(T)).