Off-line and on-line optical monitoring of microalgal growth

被引:4
|
作者
Lazcano-Hernandez, Hugo-Enrique [1 ]
Aguilar, Gabriela [2 ]
Antonia Dzul-Cetz, Gabriela [2 ]
Patino, Rodrigo [2 ]
Arellano-Verdejo, Javier [3 ]
机构
[1] Catedras CONACYT, Colegio Frontera Sur, Chetmal, Quintana Roo, Mexico
[2] Cinvestav, Dept Fis Aplicada, Unidad Merida, Merida, Yucatan, Mexico
[3] Colegio Frontera Sur, Estn Recepc Informac Satelital ERIS Chetumal, Chetmal, Quintana Roo, Mexico
来源
PEERJ | 2019年 / 7卷
关键词
Chlamydomonas reinhardtii; Chlorophyll fluorescence; Chlorophyll optical density; Fluorescence ratio; Chl a fluorescence images; C. reinhardtii cultures growth; Proximal remote sensing; CHLOROPHYLL FLUORESCENCE; PHOTOBIOREACTORS; CULTURES;
D O I
10.7717/peerj.7956
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The growth of Chlamydomonas reinhardtii microalgae cultures was successfully monitored, using classic off-line optical techniques (optical density and fluorescence) and on-line analysis of digital images. In this study, we found that the chlorophyll fluorescence ratio F685/F740 has a linear correlation with the logarithmic concentration of microalgae. By using digital images, the biomass concentration correlated with the luminosity of the images through an exponential equation and the length of penetration of a super luminescent blue beam (lambda = 440 nm) through an inversely proportional function. The outcomes of this study are useful to monitor both research and industrial microalgae cultures.
引用
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页数:17
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