Replications of Two Closely Related Groups of Jumbo Phages Show Different Level of Dependence on Host-encoded RNA Polymerase

被引：23

作者：

Matsui, Takeru ^{[1
]}

Yoshikawa, Genki ^{[2
]}

Mihara, Tomoko ^{[2
]}

Chatchawankanphanich, Orawan ^{[3
,4
]}

Kawasaki, Takeru ^{[1
]}

Nakano, Miyako ^{[1
]}

Fujie, Makoto ^{[1
]}

Ogata, Hiroyuki ^{[2
]}

Yamada, Takashi ^{[1
]}

机构：

[1] Hiroshima Univ, Grad Sch Adv Sci Matter, Dept Mol Biotechnol, Higashihiroshima, Japan

[2] Kyoto Univ, Inst Chem Res, Bioinformat Ctr, Kyoto, Japan

[3] NSTDA, Natl Ctr Genet Engn & Biotechnol, Plant Res Lab, Pathum Thani, Thailand

[4] Kasetsart Univ, Ctr Agr Biotechnol, Nakhon Pathom, Thailand

来源：

FRONTIERS IN MICROBIOLOGY | 2017年 / 8卷

关键词：

jumbo phages; Phi KZ-like phages; Ralstonia solanacearum; genomic analysis; virion-associated-RNA polymerase; COMPLETE GENOME SEQUENCE; BACTERIOPHAGE-PHI-KZ; RALSTONIA-SOLANACEARUM; VERSION;

D O I：

10.3389/fmicb.2017.01010

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Ralstonia solanacearum phages Phi RP12 and Phi RP31 are jumbo phages isolated in Thailand. Here we show that they exhibit similar virion morphology, genome organization and host range. Genome comparisons as well as phylogenetic and proteomic tree analyses support that they belong to the group of Phi KZ-related phages, with their closest relatives being R. solanacearum phages Phi RSL2 and Phi RSF1. Compared with Phi RSL2 and Phi RSF1, Phi RP12 and Phi RP31 possess larger genomes (ca. 280 kbp, 25% larger). The replication of Phi RP12 and Phi RP31 was not affected by rifampicin treatment (20 mu g/ml), suggesting that phage-encoded RNAPs function to start and complete the infection cycle of these phages without the need of host-encoded RNAPs. In contrast, Phi RSL2 and Phi RSF1, encoding the same set of RNAPs, did not produce progeny phages in the presence of rifampicin (5 mu g/ml). This observation opens the possibility that some Phi RP12/Phi RP31 factors that are absent in Phi RSL2 and Phi RSF1 are involved in their host-independent transcription.

引用

页数：11