Effect of colloidal gold size on the conformational changes of adsorbed cytochrome c:: Probing by circular dichroism, UV-visible, and infrared spectroscopy

被引:151
|
作者
Jiang, X [1 ]
Jiang, UG [1 ]
Jin, YD [1 ]
Wang, EK [1 ]
Dong, SJ [1 ]
机构
[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Peoples R China
关键词
D O I
10.1021/bm049744l
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The conformational changes of bovine heart cytochrome c (cyt c) induced by the adsorption on gold nanoparticles with different sizes have been investigated by electronic absorption, circular dichroism (CD), and Fourier transform infrared spectra. The combination of these techniques can give complementary information about adsorption-induced conformational changes. The results show that there are different conformational changes for cyt c adsorbed on gold nanoparticles with different sizes due to the different interaction forces between cyt c and gold nanoparticles. The colloidal gold concentration-dependent conformation distribution curves of cyt c obtained by analysis of CD spectra using the singular value decomposition least-squares method show that the coverage of cyt c on the gold nanoparticles surface also affects the conformational changes of the adsorbed cyt c.
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页码:46 / 53
页数:8
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