Live-cell imaging of neurofilament transport in cultured neurons

被引:10
|
作者
Uchida, Atsuko [1 ]
Monsma, Paula C. [1 ]
Fenn, J. Daniel [1 ]
Brown, Anthony [1 ]
机构
[1] Ohio State Univ, Dept Neurosci, Columbus, OH 43210 USA
基金
美国国家科学基金会;
关键词
SLOW AXONAL-TRANSPORT; INTERMEDIATE-FILAMENTS; FLUORESCENT PROTEINS; EXPRESSION; BRIGHT; RED; DIFFERENTIATION; MOVEMENT; INVITRO; ABSENCE;
D O I
10.1016/bs.mcb.2015.07.001
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Neurofilaments, which are the intermediate filaments of nerve cells, are space-filling cytoskeletal polymers that contribute to the growth of axonal caliber. In addition to their structural role, neurofilaments are cargos of axonal transport that move along microtubule tracks in a rapid, intermittent, and bidirectional manner. Though they measure just 10 nm in diameter, which is well below the diffraction limit of optical microscopes, these polymers can reach 100 mm or more in length and are often packed densely, just tens of nanometers apart. These properties of neurofilaments present unique challenges for studies on their movement. In this article, we describe several live-cell fluorescence imaging strategies that we have developed to image neurofilament transport in axons of cultured neurons on short and long timescales. Together, these methods form a powerful set of complementary tools with which to study the axonal transport of these unique intracellular cargos.
引用
收藏
页码:21 / +
页数:6
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