The expression of tumstatin is down-regulated in renal carcinoma

被引：11
|
作者：
Xu, Chun-xiao [1 ]
Liu, Xian-xi [1 ]
Hou, Guo-sheng [2 ]
Yan, Yun-fei [1 ]
Chen, Shi-min [1 ]
Wang, Wei [1 ]
Jiang, Guang-shui [3 ]
Liu, Bin [1 ]
Xin, Jia-xuan [1 ]
机构：
[1] Shandong Univ, Sch Med, Inst Biochem & Mol Biol, Jinan 250012, Shandong, Peoples R China
[2] Quangang Pharmaceut SD Co Ltd, Jinan 250014, Peoples R China
[3] Shandong Univ, Hosp Stomatol, Jinan 250012, Peoples R China
来源：
MOLECULAR BIOLOGY REPORTS | 2010年 / 37卷 / 05期
关键词：
Tumstatin; Gene expression; Angiogenesis; Renal carcinoma; DISTINCT ANTITUMOR PROPERTIES; ENDOTHELIAL GROWTH-FACTOR; IV COLLAGEN CHAINS; BASEMENT-MEMBRANE; TUMOR-GROWTH; ALPHA(V)BETA(3) INTEGRIN; ENDOGENOUS INHIBITOR; CELL CARCINOMA; ALPHA-3; CHAIN; ANGIOGENESIS;
D O I：
10.1007/s11033-009-9718-9
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Tumstatin is the 28 kDa NC1 domain of the alpha 3 chain of type IV collagen that inhibits pathological angiogenesis and suppresses endothelial cell proliferation and tumor growth. In the present paper, we expressed and purified recombinant human tumstatin protein and then prepared the anti-tumstatin polyclonal antibody. To investigate the expression of tumstatin in renal carcinoma, tumstatin protein was detected by western blotting using the prepared anti-tumstatin antibody and tumstatin mRNA levels were assayed by RT-PCR. The results showed that the expression of tumstatin gene was down-regulated in renal carcinoma tissues and cells. Our study suggests that as a novel endogenous angiogenesis inhibitor, tumstatin gene expression may be a marker for diagnosis, therapy and prognosis of renal carcinoma.
引用
收藏
页码：2273 / 2277
页数：5
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