Aptamer-Mediated Chemiluminescence Detection of Prion Protein on a Membrane Using Trimethoxyphenylglyoxal

被引：11
|
作者：
Hossain, Md Towhid [1 ]
Shibata, Takayuki [1 ]
Kabashima, Tsutomu [1 ]
Kai, Masaaki [1 ,2 ]
机构：
[1] Nagasaki Univ, Grad Sch Biomed Sci, Fac Pharmaceut Sci, Nagasaki 8528521, Japan
[2] Nagasaki Univ, Global Ctr Excellence Program, Nagasaki 8528521, Japan
来源：
ANALYTICAL SCIENCES | 2010年 / 26卷 / 06期
关键词：
REAGENT; PRPSC;
D O I：
10.2116/analsci.26.645
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Effective recognition and quantitative analysis of the prion protein are important in drug discovery and diagnosis for prion diseases, such as bovine spongiform encephalopathy and Creutzfeldt-Jakob diseases. We have developed a high-throughput method for a specific and sensitive determination of prion protein on a solid-phase membrane, based on a chemiluminescence reaction of aptamer with 3,4,5-trimethoxyphenylglyoxal. This method using aptamer is facile, inexpensive and convenient for the detection of the prion protein on a membrane, indicating a lower detection limit of ca. 4.2 pmol spot(-1).
引用
收藏
页码：645 / 647
页数：3
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