Platelet enhancement by Carica papaya L. leaf fractions in cyclophosphamide induced thrombocytopenic rats is due to elevated expression of CD110 receptor on megakaryocytes

被引：14

作者：

Nandini, C. ^{[1
]}

Madhunapantula, SubbaRao, V ^{[2
]}

Bovilla, Venugopal R. ^{[2
]}

Ali, Mohammad ^{[1
]}

Mruthunjaya, K. ^{[3
]}

Santhepete, Manjula N. ^{[1
]}

Jayashree, K. ^{[4
]}

机构：

[1] JSS Acad Higher Educ & Res, JSS Coll Pharm, Dept Pharmacol, Mysuru 570015, Karnataka, India

[2] JSS Acad Higher Educ & Res, JSS Med Coll, Ctr Excellence Mol Biol & Regenerat Med CEMR, Mysuru, Karnataka, India

[3] JSS Acad Higher Educ & Res, JSS Coll Pharm, Dept Pharmacognosy, Mysuru, Karnataka, India

[4] JSS Acad Higher Educ & Res, JSS Med Coll & Hosp, Dept Pathol, Mysuru, Karnataka, India

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 2021年 / 275卷

关键词：

Thrombocytopenia; Thrombopoietin; CD110/cMpl receptor; Carica papaya leaf; Megakaryocytes; CHEMOTHERAPY-INDUCED THROMBOCYTOPENIA; BONE-MARROW; INDUCED HEPATOTOXICITY; THROMBOPOIETIN LEVELS; PROTHROMBIN TIME; BLEEDING-TIME; DENGUE-FEVER; C-MPL; LEAVES; LIVER;

D O I：

10.1016/j.jep.2021.114074

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Ethnopharmacological relevance: Carica papaya leaf juice/decoction has been in use in folk medicine in Srilanka, Malaysia and in few parts of India for enhancing the platelet counts in dengue. In Siddha medicine, a traditional form of medicine in India, papaya leaf juice has been used for increasing the platelet counts. Papaya leaf has been reported to enhance blood volume in ancient Ayurveda books in India. Carica papaya leaf is well known for its platelet enhancement activity. Although many preclinical and clinical studies have demonstrated the ability of papaya leaf juice for platelet enhancement, but the underlying mechanisms are still unclear. Aim of the study: The study is aimed at identifying the key ingredients of papaya leaf extract and elucidate the mechanism (s) of action of the identified potent component in mitigating thrombocytopenia (Thp). Materials and methods: C. papaya leaf juice was subjected for sequential fractionation to identify the anti-thrombocytopenic phytochemicals. In vivo, stable thrombocytopenia was induced by subcutaneous injection of 70 mg/kg cyclophosphamide (Cyp). After induction, rats were treated with 200 and 400 mg/kg body weight papaya leaf juice and with identified fractions for 14 days. Serum thrombopoietin level was estimated using ELISA. CD110/cMpl, a receptor for thrombopoietin on platelets was measured by western blotting. Results: Administration of cyclophosphamide for 6 days induced thrombocytopenia (210.4 +/- 14.2 x 10(3) cells/mu L) in rats. Treating thrombocytopenic rats with papaya leaf juice and butanol fraction for 14 days significantly increased the platelet count to 1073.50 +/- 29.6 and 1189.80 +/- 36.5 x 10(3) cells/mu L, respectively. C.papaya extracts normalized the elevated bleeding and clotting time and decreased oxidative markers by increasing endogenous antioxidants. A marginal increase in the serum thrombopoietin (TPO) level was observed in Cyp treated group compared to normal and treatment groups. Low expression of CD110/cMpl receptor found in Cyp treated group was enhanced by C. papaya extracts (CPJ) and CPJ-BT. Furthermore, examination of the morphology of bone marrow megakaryocytes, histopathology of liver and kidneys revealed the ability of CPJ and fractions in mitigating Cyp-induced thrombocytopenia in rats. Conclusion: C. papaya leaf juice enhances the platelet count in chemotherapy-induced thrombocytopenia by increasing the expression of CD110 receptor on the megakaryocytes. Hence, activating CD110 receptor might be a viable strategy to increase the platelet production in individuals suffering from thrombocytopenia.

引用

页数：16