In situ study of ligand-receptor interaction by total internal reflection ellipsometry

被引：28
|
作者：
Balevicius, Z. [1 ,3 ]
Baleviciute, I. [2 ]
Tumenas, S. [1 ]
Tamosaitis, L. [1 ,2 ]
Stirke, A. [1 ]
Makaraviciute, A. [1 ,2 ]
Ramanaviciene, A. [2 ]
Ramanavicius, A. [1 ,2 ]
机构：
[1] State Res Inst, Ctr Phys Sci & Technol, Savanoriu Ave 231, LT-01108 Vilnius, Lithuania
[2] Vilnius Univ, Fac Chem, LT-03225 Vilnius, Lithuania
[3] Vilnius Gediminas Tech Univ, Fac Elect, LT-10223 Vilnius, Lithuania
来源：
THIN SOLID FILMS | 2014年 / 571卷
关键词：
In situ spectroscopic ellipsometry; SPR enchanced ellipsometry; Total internal reflection ellipsometry; Ligand-receptor interaction; Granulocyte colony stimulating factor (GCSF); ANTIBODY IMMOBILIZATION TECHNIQUES; PHASE;
D O I：
10.1016/j.tsf.2013.10.090
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
Spectroscopic total internal reflection ellipsometry (TIRE) in its dynamic data acquisition mode has been applied for the in situ study of the interactions of monomeric (mGCSF) and dimeric (dGCSF) granulocyte colony stimulating factor and its receptor (GCSF-R) which was immobilized on a thin gold layer. Regression results have shown that the account for signal non-linearity due to analysis of spectroscopic dynamic TIRE data has allowed us to detect small differences in association and dissociation times through the precise determination of the refractive index dispersion. Different binding models were applied in the study of mGCSF and dGCSF interaction with a monolayer of its receptor GCSF-R due to their distinctive changes of refractive index over time. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码：744 / 748
页数：5
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