Step-Wise Refolding of Recombinant Proteins

被引：12
|
作者：
Tsumoto, Kouhei [1 ]
Arakawa, Tsutomu [2 ]
Chen, Linda [3 ]
机构：
[1] Univ Tokyo, Grad Sch Frontier Sci, Kashiwa, Chiba 2778562, Japan
[2] Alliance Prot Labs, Thousand Oaks, CA 91360 USA
[3] Life Technol, Camarillo, CA 93012 USA
来源：
CURRENT PHARMACEUTICAL BIOTECHNOLOGY | 2010年 / 11卷 / 03期
关键词：
Step-wise refolding; arginine; aggregation; antibody fragment; dialysis; CHAIN FV FRAGMENT; ESCHERICHIA-COLI; INCLUSION-BODIES; IN-VITRO; EFFECTIVE RENATURATION; ARGININE; AGGREGATION;
D O I：
10.2174/138920110791111979
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Protein refolding is still on trial-and-error basis. Here we describe step-wise dialysis refolding, in which denaturant concentration is altered in step-wise fashion. This technology controls the folding pathway by adjusting the concentrations of the denaturant and other solvent additives to induce sequential folding or disulfide formation.
引用
收藏
页码：285 / 288
页数：4
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