An efficient protocol for in vitro regeneration of Podophyllum hexandrum, a critically endangered medicinal plant

被引：0

作者：

Chakraborty, Amrita ^{[1
]}

Bhattacharya, Dipto ^{[1
]}

Ghanta, Srijani ^{[1
]}

Chattopadhyay, Sharmila ^{[1
]}

机构：

[1] Indian Inst Chem Biol CSIR, Kolkata 700032, India

来源：

INDIAN JOURNAL OF BIOTECHNOLOGY | 2010年 / 9卷 / 02期

关键词：

Podophyllum hexandrum; rhizome explants; endangered medicinal plant; conservation; in vitro propagation; podophyllotoxin; PRURIENS-F-PRURIENS; TISSUE-CULTURES; ACTIVATED-CHARCOAL; CELL-CULTURES; GROWTH; LIGNANS; MORPHOGENESIS; PROPAGATION; PELTATUM; TOBACCO;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Rhizome explants were used to develop a protocol for in vitro plantlet regeneration of Podophyllum hexandrum, a critically endangered medicinal plant, through direct organogenesis. Highest rate of multiple shoot formation was noted in MS medium supplemented with 11.42 mu M IAA within three months. A synergistic effect of 2.68 mu M NAA and 11.1 mu M BAP was second best. The 1/2 MS liquid medium with 100 mu M IBA was most suitable for rooting of shoots. Leaf explants resulted in callus formation only. Leaf regenerated calli showed the presence of podophyllotoxin in HPLC analysis.

引用

页码：217 / 220

页数：4

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