Evidence that the tumor-suppressor protein BRCA2 does not regulate cytokinesis in human cells

被引:27
|
作者
Lekomtsev, Sergey [1 ,2 ]
Guizetti, Julien [3 ]
Pozniakovsky, Andrei [4 ]
Gerlich, Daniel W. [3 ]
Petronczki, Mark [1 ,2 ]
机构
[1] Canc Res UK London Res Inst, Clare Hall Labs, Cell Div, S Mimms EN6 3LD, Herts, England
[2] Canc Res UK London Res Inst, Clare Hall Labs, Aneuploidy Lab, S Mimms EN6 3LD, Herts, England
[3] Swiss Fed Inst Technol Zurich ETHZ, Inst Biochem, CH-8093 Zurich, Switzerland
[4] Max Planck Inst Mol Cell Biol & Genet, D-01307 Dresden, Germany
关键词
BRCA2; Cytokinesis; Chromosomal instability; Cell division; Mitosis; CANCER SUSCEPTIBILITY; INHIBITOR; MUTATIONS; RAD51;
D O I
10.1242/jcs.068015
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Germline mutations in the tumor-suppressor gene BRCA2 predispose to breast and ovarian cancer. BRCA2 plays a well-established role in maintaining genome stability by regulating homologous recombination. BRCA2 has more recently been implicated in cytokinesis, the final step of cell division, but the molecular basis for this remains unknown. We have used time-lapse microscopy, recently developed cytokinesis assays and BAC recombineering (bacterial artificial chromosome recombinogenic engineering) to investigate the function and localization of BRCA2 during cell division. Our analysis suggests that BRCA2 does not regulate cytokinesis in human cells. Thus, cytokinesis defects are unlikely to contribute to chromosomal instability and tumorigenesis in BRCA2-related cancers.
引用
收藏
页码:1395 / 1400
页数:6
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