Identification of the JY-1 in sika deer and its effects on granulosa cells cultured in vitro

被引：0
|
作者：
Chen, Xiumin [1 ,2 ]
Xing, Mingjie [1 ,2 ]
Yang, Yifeng [1 ,2 ]
Chen, Min [1 ,2 ]
Chang, Tong [1 ,2 ]
Xu, Baozeng [1 ,2 ]
Wei, Haijun [1 ,2 ]
机构：
[1] Chinese Acad Agr Sci, Inst Special Anim & Plant Sci, Changchun, Jilin, Peoples R China
[2] Key Lab Special Econ Anim Mol Biol, Changchun, Jilin, Peoples R China
来源：
REPRODUCTION IN DOMESTIC ANIMALS | 2019年 / 54卷 / 11期
基金：
国家重点研发计划;
关键词：
apoptosis; cell cycle; JY-1; MESSENGER-RNA ABUNDANCE; OOCYTE-SPECIFIC GENE; CDNA LIBRARY; BOVINE JY-1; EXPRESSION;
D O I：
10.1111/rda.13471
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
The oocyte-specific protein JY-1 was reported as an important regulator of both function of ovarian granulosa cells and early embryogenesis in cattle. Here, we found that the transcripts of JY-1 were also present in sika deer granulosa cells (GCs) through in situ hybridization and qRT-PCR. The complete sika deer JY-1 coding sequence was identified, which had three exons separated by two introns. It was detected that JY-1 knockdown caused apoptosis and abnormal cell cycle progression in GCs of sika deer cultured in vitro. Taken together, these data suggest that JY-1 is involved in the regulation of proliferation in sika deer ovarian GCs.
引用
收藏
页码：1437 / 1442
页数：6
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