Synthesis and characterization of a cellular membrane affinity chromatography column containing histamine 1 and P2Y1 receptors: A multiple G-protein coupled receptor column

被引：16

作者：

Moaddel, Ruin ^{[1
]}

Musyimi, Harrison K. ^{[1
]}

Sanghvi, Mitesh ^{[1
]}

Bashore, Charlene ^{[1
]}

Frazier, Chester R. ^{[1
]}

Khadeer, Mohammad ^{[1
]}

Bhatia, Prateek ^{[1
]}

Wainer, Irving W. ^{[1
]}

机构：

[1] NIA, NIH, Gerontol Res Ctr, Baltimore, MD 21224 USA

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2010年 / 52卷 / 03期

关键词：

Affinity chromatography; Drug discovery; Receptor screening; Anti-histamines; Membrane receptors; STATIONARY PHASES;

D O I：

10.1016/j.jpba.2009.06.035

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A cellular membrane affinity chromatography (CMAC) column has been created using cellular membrane fragments from a 1321N1 cell line stably transfected with the P2Y(1) receptor. The CMAC(1321N1(P2Y1)) column contained functional P2Y(1) and histamine I receptors, which independently bound receptor-specific ligands. The data obtained with the CMAC(1321N1(P2Y1)) column demonstrate that multiple-G-protein coupled receptor (GPCR) columns can be developed and used to probe interactions with the immobilized receptors and that endogenously expressed GPCRs can be used to create CMAC columns. The results also establish that the histamine 1 receptor can be immobilized with retention of ligand-specific binding. Published by Elsevier B.V.

引用

页码：416 / 419

页数：4

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