Chemiluminescence-HPLC for the assay of guanidino compounds

被引：0
|
作者：
Nohara, Y [1 ]
Hanai, T
Kinoshita, T
Watanabe, M
机构：
[1] Teikyo Univ, Fac Pharmaceut Sci, Kanagawa 1990195, Japan
[2] Hlth Res Fdn, Int Inst Technol Anal, Sakyo Ku, Kyoto 606, Japan
[3] Kitasato Univ, Sch Pharmaceut Sci, Minato Ku, Tokyo 1088641, Japan
来源：
CHEMICAL & PHARMACEUTICAL BULLETIN | 2000年 / 48卷 / 11期
关键词：
guanidino compound; chemiluminescence; ninhydrin; lucigenin; HPLC; chronic renal failure;
D O I：
暂无
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
A chemiluminescence-HPLC system for the assay of serum guanidino compounds was established. In this study; we found that lucigenin added to the reaction mixture containing guanidino compounds and alkaline-ninhydrin reagent resulted in strong chemiluminescence. The lucigenin chemiluminescence was not derived from fluorescent derivatives of guanidino compounds or ninhydrin. The interaction of guanidino compounds and ninhydrin under alkaline conditions produced reactive oxygen species. We applied this phenomenon to the microanalysis of guanidino compounds.
引用
收藏
页码：1841 / 1842
页数：2
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