With the development of commercial services in the European Union, the advanced designs of Galileo E6 signals can be freely offered to Global Navigation Satellite System (GNSS) users. However, only a few studies have analyzed the benefit of Galileo E6 signals, and their application in the real-time instantaneous precise point positioning (PPP) with ambiguity resolution (AR) has not been investigated. In this study, based on the triple-frequency ionosphere-free combination, the positioning performance of instantaneous decimeter-level PPP AR was assessed with real-time orbit, clock and phase biases provided by the Centre National d'Etudes Spatiales Navigation Team (CNES/NAV). Experimenting on the GPS/Galileo observations from the 42 globally distributed stations, the wide-lane (WL) ambiguity residuals within +/- 0.25 cycle for GPS L1/L2/L5, Galileo E1/E5a/E5b and E1/E5a/E6 frequency combinations were 92.54%, 90.72% and 96.48% with single-epoch data, respectively. When the WL ambiguities are fixed instantaneously, owing to the low combination noise of Galileo E1/E5a/E6, the positioning performance of instantaneous PPP AR with GPS/Galileo (E1/E5a/E6 used) outperforms that of GPS/Galileo (E1/E5a/E5b used). If the position dilution of precision is <1.5, the Root Mean Square (RMS) of GPS/Galileo (E1/ E5a/E6 used) for east, north and up components are 0.13 m, 0.13 m and 0.57 m, respectively, while those of GPS/Galileo (E1/E5a/ E5b used) are 0.17 m, 0.19 m and 0.62 m, respectively. Moreover, the WL ambiguity fixing rate of GPS/Galileo (E1/E5a/E6 used) is higher than that of GPS/Galileo (E1/E5a/E5b used), which shows an improvement of approximately 9% in the relatively low satellite elevation angles (20 degrees< elevation angle < 40 degrees). The results indicate that the Galileo E6 signal can bring a significant improvement in real-time instantaneous decimeter-level PPP AR. (C) 2022 COSPAR. Published by Elsevier B.V. All rights reserved.