Microdroplets in Microfluidics: An Evolving Platform for Discoveries in Chemistry and Biology

被引：863

作者：

Theberge, Ashleigh B. ^{[1
]}

Courtois, Fabienne ^{[1
,2
]}

Schaerli, Yolanda ^{[1
,2
]}

Fischlechner, Martin ^{[1
]}

Abell, Chris ^{[1
]}

Hollfelder, Florian ^{[2
]}

Huck, Wilhelm T. S. ^{[1
,3
]}

机构：

[1] Univ Cambridge, Dept Chem, Lensfield Rd, Cambridge CB2 1EW, England

[2] Univ Cambridge, Dept Biochem, Cambridge CB2 1GA, England

[3] Radboud Univ Nijmegen, Inst Mol & Mat, NL-6525 AJ Nijmegen, Netherlands

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 34期

基金：

英国工程与自然科学研究理事会;

关键词：

biotechnology; emulsions; microfluidics; self-assembly; surfactants; POLYMERASE-CHAIN-REACTION; SINGLE-COPY DNA; ON-CHIP; REAL-TIME; ELECTROCHEMICAL DETECTION; MULTIPHASE MICROFLUIDICS; MICROFABRICATED CHANNEL; SUBCELLULAR ORGANELLES; CAPILLARY-MICROREACTOR; RAMAN-SPECTROSCOPY;

D O I：

10.1002/anie.200906653

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Microdroplets in microfluidics offer a great number of opportunities in chemical and biological research. They provide a compartment in which species or reactions can be isolated, they are monodisperse and therefore suitable for quantitative studies, they offer the possibility to work with extremely small volumes, single cells, or single molecules, and are suitable for high-throughput experiments. The aim of this Review is to show the importance of these features in enabling new experiments in biology and chemistry. The recent advances in device fabrication are highlighted as are the remaining technological challenges. Examples are presented to show how compartmentalization, monodispersity, single-molecule sensitivity, and high throughput have been exploited in experiments that would have been extremely difficult outside the microfluidics platform. © 2010 wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：5846 / 5868

页数：23

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