Genetic variation within and among rainbow trout, Onchorhynchus mykiss, hatchery populations from Iran assessed by PCR-RFLP analysis of mitochondrial DNA segments

Samples were collected from 3 hatchery populations of 2 trout inbreeding centers (Kelardasht and Jajrood) and mitochondrial DNA (mtDNA) of these samples was studied by polymerase chain reaction-restriction fragment length polymorphism. (PCR-RFLP). ND5/6 segment and a fragment that includes 12SrRNA, tRNA F, P, T, D-loop, and a part of cyt.b of mtDNA were amplified and investigated using different restriction enzymes. Two patterns were observed using HaeIII, AvaII, RsaI, TaqI, and HinfI enzymes on gel electrophoresis, resulting in 9 haplotypes in ND5/6 segment, whereas only 1 pattern was observed for the fragment containing D-loop. As a measure of intrapopulation diversity and interpopulation diversity, both heterozygocity and genetic distance were determined, respectively.