Enzymatic determination of uric acid using water-soluble CuInS/ZnS quantum dots as a fluorescent probe

被引：38
|
作者：
Zhang, Fangmei [1 ,2 ]
Ma, Pinyi [1 ]
Deng, Xinyu [1 ]
Sun, Ying [1 ]
Wang, Xinghua [1 ]
Song, Daqian [1 ]
机构：
[1] Jilin Univ, Coll Chem, Qianjin St 2699, Changchun 130012, Jilin, Peoples R China
[2] Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Harbin 150070, Heilongjiang, Peoples R China
来源：
MICROCHIMICA ACTA | 2018年 / 185卷 / 11期
基金：
中国国家自然科学基金;
关键词：
Microwave-based synthesis; Nanoparticle synthesis; Fluorescent probe; Uric acid determination; Human urine and serum; ENHANCED RAMAN-SCATTERING; QUANTIFICATION; SAMPLES; RISK;
D O I：
10.1007/s00604-018-3030-0
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Glutathione-capped water-soluble CuInS/ZnS quantum dots (QDs) were prepared by a microwave-assisted method. Their fluorescence, with excitation/emission peaks at 380/570 nm, is found to be quenched by hydrogen peroxide (H2O2) that is produced by the ukase catalyzed oxidation of uric acid (UA) and oxygen. The findings are used in a quenchometric method for the determination of UA. The effects of different ligands on the QDs, of pH value, buffers, enzyme ratio and reaction time were optimized. The detection limit for UA is 50 nM which is lower than other QD-based method, and the detection ranges extends from 0.25-4.0 mu M. The assay is simple and sensitive, and no further modification of the QDs is required.
引用
收藏
页数：8
相关论文
共 50 条
  • [1] Enzymatic determination of uric acid using water-soluble CuInS/ZnS quantum dots as a fluorescent probe
    Fangmei Zhang
    Pinyi Ma
    Xinyu Deng
    Ying Sun
    Xinghua Wang
    Daqian Song
    [J]. Microchimica Acta, 2018, 185
  • [2] Quantitative determination of pazufloxacin using water-soluble quantum dots as fluorescent probes
    Ling Xia
    Deng Da-wei
    Zhong Wen-ying
    Yu Jun-sheng
    [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28 (06) : 1317 - 1321
  • [3] Water-soluble ZnO fluorescent quantum dots
    Fu Ying-song
    Du Xi-wen
    Qiu Jian-sheng
    Li Rui
    Liu Jian-yong
    [J]. JOURNAL OF CENTRAL SOUTH UNIVERSITY OF TECHNOLOGY, 2007, 14 : 16 - 19
  • [4] A Review, Water-Soluble CuInS Quantum Dots, Strategies and Photoluminescence
    Kamel, Ola A. A.
    Fouad, M.
    Ali, Magdy
    [J]. INTERNATIONAL JOURNAL OF NANOSCIENCE, 2023, 22 (01)
  • [5] Temperature-dependent photoluminescence properties of water-soluble CuInS2 and CuInS2/ZnS quantum dots
    Iida, Kazutaka
    Kim, DaeGwi
    [J]. JOURNAL OF APPLIED PHYSICS, 2022, 132 (19)
  • [6] Temperature-dependent photoluminescence properties of water-soluble CuInS2and CuInS2/ZnS quantum dots
    Iida, Kazutaka
    Kim, Daegwi
    [J]. Journal of Applied Physics, 2022, 132 (19):
  • [7] Stable water-soluble CdSe/ZnS quantum dots with higher quantum efficiency as fluorescent biological label
    Song, Z.
    Shen, Y.
    Yan, H.
    You, C.
    Yu, B.
    Zhang, J.
    Zhang, X.
    [J]. PROCEEDINGS OF THE INSTITUTION OF MECHANICAL ENGINEERS PART N-JOURNAL OF NANOMATERIALS NANOENGINEERING AND NANOSYSTEMS, 2008, 222 (03) : 129 - 133
  • [8] Water-soluble near-infrared CuInS2/ZnS fluorescent quantum dots for Cu2+detection and cellular imaging
    Wu, Jie
    Li, Jinhua
    Li, Li
    Dong, Wen-Fei
    Jiang, Chenyu
    Zhang, Fan
    [J]. MICROCHEMICAL JOURNAL, 2024, 199
  • [9] Use of water-soluble PbS quantum dots as fluorescent probe in sensing copper(II)
    Li, Ting
    Wang, Nanxi
    Chen, Lijia
    Deng, Dawei
    Gu, Yueqing
    [J]. ADVANCED SENSOR SYSTEMS AND APPLICATIONS IV, 2010, 7853
  • [10] Water-soluble MoS2 quantum dots are a viable fluorescent probe for hypochlorite
    Wang, Yi
    Zhang, Pu
    Lu, Qing
    Wang, Yue
    Fu, Wensheng
    Tan, Qin
    Luo, Weiping
    [J]. MICROCHIMICA ACTA, 2018, 185 (04)
12345