Improvement and Interlaboratory Validation of the Lipophilic Oxygen Radical Absorbance Capacity: Determination of Antioxidant Capacities of Lipophilic Antioxidant Solutions and Food Extracts

被引：15
|
作者：
Watanabe, Jun [1 ]
Oki, Tomoyuki [2 ]
Takebayashi, Jun [3 ]
Yada, Hiroshi [1 ]
Wagaki, Manabu [1 ]
Takano-Ishikawa, Yuko [1 ]
Yasui, Akemi [1 ]
机构：
[1] Natl Agr & Food Res Org, Natl Food Res Inst, 2-1-12 Kannondai, Tsukuba, Ibaraki 3058642, Japan
[2] Natl Agr & Food Res Org, Natl Agr Res Ctr Kyushu Okinawa Reg, 2421 Suya, Koshi, Kumamoto 861192, Japan
[3] Natl Inst Hlth & Nutr, Natl Inst Biomed Innovat Hlth & Nutr, Shinjuku Ku, 1-23-1 Toyama, Tokyo 1628636, Japan
来源：
ANALYTICAL SCIENCES | 2016年 / 32卷 / 02期
关键词：
Lipophilic oxygen radical absorbance capacity; antioxidant capacity; method validation; ASSAY;
D O I：
10.2116/analsci.32.171
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A lipophilic oxygen radical absorbance capacity (L-ORAC) assay is an evaluation of the antioxidant capacity of solutions of lipophilic compounds. The concentrations of fluorescein, radical generator, and Trolox standard solutions were optimized to improve the precision of the assay. An interlaboratory study using two antioxidant solutions and three food extracts as test samples conducted in accordance with harmonized protocol demonstrated satisfactory L-ORAC measurements; the intermediate precision relative standard deviations (RSDmt) ranged from 7.0 to 16.7%, the reproducibility relative standard deviations (RSDR) ranged from 14.8 to 19.4%, and the HorRat values ranged from 1.35 to 1.78.
引用
收藏
页码：171 / 175
页数：5
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