Effect of miR-143 on proliferation of osteosarcoma cells under low dosage cisplatin

Osteosarcoma is one primary malignant bone tumor that commonly occurs in children and teenagers. Its high malignancy, invasiveness and metastasis all lead to unfavorable prognosis even with combined treatment. Cisplatin is a common chemotherapy agent in treating various solid tumors including ovarian, pulmonary, nasopharyngeal, esophageal, thyroid cancers and osteosarcoma, but leaving its mechanism largely unclear. Micro RNA (miRNA) has been suggested to have abnormal expression in tumor tissues. This study thus observed the effect of miR-143 on the proliferation of osteosarcoma cell MG63, via the manipulation of miR-143 expression under the exposure of low dosage cisplatin. CCK8 assay was performed to observe the influence of cisplatin on MG63 cell proliferation to determine LC50 value of cisplatin. MG63 osteosarcoma cells were ten transfected with miR-143 mimic or miR-143 inhibitor to observe the altered cell proliferation under low concentration of cisplatin exposure. Cisplatin could inhibit proliferation of MG63 cells. Under low dosage cisplatin, expression of miR-143 increased. The manipulation of endogenous miR-143 level can affect the proliferation of osteosarcoma cells. The up-regulation of miR-143 can inhibit the proliferation of osteosarcoma. Under the exposure of low dosage of cisplatin, miR-143 may participate in the inhibition of tumor cell proliferation.