Fabrication, characterization, and biological assessment of multilayer laminin γ2 DNA coatings on titanium surfaces

The purpose of this work was to fabricate a multilayer laminin gamma 2 DNA coating on a titanium surface and evaluate its biological properties. A multilayer laminin gamma 2 DNA coating was fabricated on titanium using a layer-by-layer assembly technique. The rate of coating degradation was evaluated by detecting the amount of cDNA remaining. Surface analysis using X-ray photoelectron spectroscopy, atomic force microscopy, and surface contact angle measurements revealed the multilayer structure to consist of cationic lipid and confirmed that a laminin gamma 2 DNA layer could be fabricated on titanium via the layer-by-layer assembly process. The transfection efficiency was highest for five layers in the multilayer structure. HEK293 cells cultured on the multilayer films displayed significantly higher adhesion activity than the control group. The expression of laminin gamma 2 and the co-localization of integrin beta 4 and plectin were more obvious in HN4 cells cultured on the multilayer laminin gamma 2 DNA coating, while weak immunoreactivities were observed in the control group. We concluded that the DNA-loaded multilayer provided a surface with good biocompatibility and that the multilayer laminin gamma 2 DNA coating might be effective in improving cell adhesion and the formation of hemidesmosomes on titanium surfaces.